Relying on the Guangzhou Baiyun Airport Underpass Tunnel Project, the impact of the underpass tunnel construction on the aircraft runway and the impact of aircraft loading above on the operation of the underpass tunnel are analyzed. Three?dimensional finite element simulation is used to analyze the results of the strata, the maximum displacement of the segments and the maximum stress of the segments during the most unfavorable situation, namely the landing and taxiing process of the aircraft directly above the tunnel. The results show that when the thickness of the overburden soil layer is relatively small, the tensile stress on the tunnel segments and the runway settlement are greatly affected by the aircraft load. The closer the landing site of the aircraft is to directly above the tunnel, the greater the impact of the aircraft load on the underpass. With the increase of the distance between the landing position and the channel axis, the tensile stress of the segment, the settlement of the stratum and the runway first increase and then decrease, reaching the maximum value when the landing position is directly above the left line tunnel.