Taking Ningbo Zhongxing Bridge as the background, the research on the stress performance of the rotary shaft during the vertically rotating construction of the V-type low-pylon cable-stayed bridge is carried out. In the process of vertically rotating construction, the rotary shaft withstands large concentrated load, which is easy to produce damage and threaten the safety of bridge construction. And the stress performance of V-type bridge pylon is even more complicated during the asymmetric rotating construction. The overall calculation model of the pylon during the vertically rotating construction is established to analyze the most unfavorable construction state of concentrated load effect. According to the most unfavorable construction state, the partial analysis model of vertically rotary shaft is established to analyze its partial stress performance. The results show that the strength and stiffness of the rotary shafts can meet the safety requirements during the vertically rotating construction of the V-type pylon.