The measured strain value of concrete structure consists of temperature strain, stress strain, shrinkage strain and creep strain, etc. It is invaluable for bridge stress evaluation and construction control to obtain the strain due to structural stress from the measured strain. Based on the measured data of concrete strain and temperature obtained by continuous synchronous monitoring, an elimination method of temperature strain was proposed. This method can make the monitoring strain accurately reflect the real stress state of the structure. And was applied and verified in a crossing-aboveSbridge in Ningbo Rail Transit Line 4, that across over Hangzhou-Shenzhen railway and Xiaoshan-Ningbo railway. It provided the basis for structural stress analysis and construction control.